Phytohormonal regulation of in vitro formation of wheat androgenic structures

Abstract

This research is devoted to developing a method of phytohormonal regulation of in vitro formation of a certain type of wheat androgenic structures. Using the method of ELISA it was shown that the induction of certain sporophytic morphogenesis pathway in vitro of anther haploid cells - microspores depends on both the content of endogenous auxin IAA in anthers before inoculating them onto induction medium, and the concentration of exogenous auxin 2,4-D in this medium. The obtained data confirms the principle possibility of regulation of ways of getting androgenic regenerants in vitro by selecting the optimal balance of endogenous and exogenous auxins