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Please use this identifier to cite or link to this item: http://dspace.bsu.edu.ru/handle/123456789/42476
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dc.contributor.authorMutsenko, V.-
dc.contributor.authorAriana Barlic-
dc.contributor.authorPezic, T.-
dc.contributor.authorJanja Dermol-Cerne-
dc.contributor.authorKatkov, I. I.-
dc.date.accessioned2021-06-28T07:44:37Z-
dc.date.available2021-06-28T07:44:37Z-
dc.date.issued2019-
dc.identifier.citationMe₂SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars / V. Mutsenko [et al.] // Cryobiology. - 2019. - Vol.91.-P. 104-114. - Doi: https://doi.org/10.1016/j.cryobiol.2019.10.002.ru
dc.identifier.urihttp://dspace.bsu.edu.ru/handle/123456789/42476-
dc.description.abstractCryopreservation is the universal technology used to enable long-term storage and continuous availability of cell stocks and tissues for regenerative medicine demands. The main components of standard freezing media are dimethyl sulfoxide (hereinafter Me₂SO) and fetal bovine serum (FBS). However, for manufacturing of cells and tissue-engineered products in accordance with the principles of Good Manufacturing Practice (GMP), current considerations in regenerative medicine suggest development of Me₂SO- and serum-free biopreservation strategies due to safety concerns over Me2SO-induced side effects and immunogenicity of animal serum. In this work, the effect of electroporation-assisted pre-freeze delivery of sucrose, trehalose and raffinose into human umbilical cord mesenchymal stem cells (hUCMSCs) on their post-thaw survival was investigatedru
dc.language.isoenru
dc.subjectbiologyru
dc.subjectdevelopmental biologyru
dc.subjectMe2SO- and serum-free cryopreservationru
dc.subjectelectroporationru
dc.subjectpermeabilizationru
dc.subjectsugarru
dc.subjecthuman umbilical cordru
dc.subjectmesenchymal stem cellsru
dc.titleMe₂SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugarsru
dc.typeArticleru
Appears in Collections:Статьи из периодических изданий и сборников (на иностранных языках) = Articles from periodicals and collections (in foreign languages)

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